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See Example V-IM-17 in the article on Imperatives for 5 ways to say no.
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A negative is a grammatical feature used to indicate that something is false (i.e., the opposite of affirmative).
καὶ μὴ λυπεῖτε τὸ πνεῦμα τὸ ἅγιον τοῦ θεοῦ (Eph 4:30)
and do not grieve the Holy Spirit of God
See Example V-IM-17 in the article on Imperatives for 5 ways to say no.