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MITYLENE
Main city on the island of Lesbos in the Aegean Sea near the northwestern coast of Asia Minor. Mitylene was a seaport with two harbors. Originally it had been built on a small island separate from Lesbos. In NT times it was connected with the main island by a raised roadway across a narrow stretch of water. Acts 20:14 identifies Mitylene as one of the overnight stopping places where Paul and his traveling companions lodged as they journeyed by ship toward Jerusalem.
Mitylene
On his third missionary journey Paul stopped at Mitylene before going on to Miletus. In Miletus he summoned the elders of the Ephesian church to say farewell to them, because he knew he would probably not see them again.